他莫昔酚对大鼠前列腺增生动物模型的抑制作用及机理研究

An Experimental Study of the Inhibition of Tamoxifen on Rat Model of Benign Prostatic Hyperplasia

目的 :探讨他莫昔酚抑制前列腺增生的作用机理。 方法 :将Wistar成年大鼠肌肉注射丙酸睾丸酮 ,同时以他莫昔酚常规灌胃。于给药 7、15、30d时分批处死大鼠 ,称取前列腺重量 ,标本做常规病理切片 ,电镜观察前列腺组织细胞结构变化。 结果 :给药 7d时单用睾酮组前列腺指数高于对照组 ,也高于灌胃组。剩余大鼠分 2组 ,其中 1组继续单用他莫昔酚灌胃 ,于第 15、30d时分组处死剩余大鼠。光镜染色观察及电镜扫描均证实他莫昔酚组前列腺上皮细胞及基质的增殖被抑制。 结论 :他莫昔酚通过对雌激素的拮抗作用 ,阻断雌激素在前列腺增生中的作用 ,从而抑制前列腺增生。

Objectives: To study the inhibitory mechanism of tamoxifen on benign prostatic hyperplasia. Methods: The Wistar male adult rats were injected into muscle with testosterone propionate 4～6 mg/kg,simultaneously were irrigated into stomach with tamoxifen citrate 0.21 mg/kg. The partly rats of each group were decapitated at 7?15 and 30 days, then their index numbers of prostate were calculated,and the structural changes of the prostatic histocyte were observed in the light microscopy and scan electronic microscopy. Results: At the 7th ?15th?and 30th day, the index numbers of prostate of those rats which had been injected only with testosterone propionate were higher than that of the control group and the irrigating group( P <0.05).The hyperplasia of the prostatic epithelial cells and the ground substance of those rats, which had been irrigated with tamoxifen citrate, had not happened in light microscopy and the scan electronic microscopy. Conclusions: Tamoxifen could block the effect of the estrogen, which could suppress the prostatic hyperplasia. This study could provide the experimental evidences for using Tamoxifen to treat the human benign prostatic hyperplasia.