HPLC法快速测定人参提取物中7种人参皂苷含量

Fast Determination of Seven Ginsenosides in Abstract of Panax Ginseng by HPLC

该文采用Kinetex核-壳色谱柱测定人参提取物中7种人参皂苷的含量。用甲醇超声波提取法提取样品,采用Phenomenex KinetexC18100A(50mm×4.6mm,2.6μm)色谱柱,柱温常温,流动相乙腈:水,梯度洗脱,流速为1.8mLmin,检测波长为205nm,柱温为室温,对人参皂苷Rg1、Re、Rf、Rb1、Rc、Rb2和Rd进行定量分析。结果发现人参皂苷Rg1、Re、Rf、Rb1、Rc、Rb2和Rd的线性良好,重复性试验中人参皂苷Rg1、Re、Rf、Rb1、Rc、Rb2和Rd相对标准偏差为2.5%、2.9%、3.8%、1.5%、3.6%、1.9%和2.3%,人参皂苷Rg1、Re、Rf、Rb1、Rc、Rb2和Rd的加标回收率分别为102.6%、99.6%、98.7%、101.3%、99.2%、98.5%和101.8%。试验证明本方法快速、节省试剂,可用于人参提取物中人参皂苷Rg1、Re、Rf、Rb1、Rc、Rb2和Rd的含量测定。

The method for the determination of seven ginsenosides in Abstract of Panax ginseng by Kinetex core-shell column was established.The separation was performed on Phenomenex Kinetex C18 100A(50 mm×4.6 mm,2.6μm).The mobile phase consisted of acetonitrile(A)-water(B)with Linear gradient elution;at a flow rate of 1.8mL·min-1:the warelength of detector was 205nm.The column temperature was at room temperature.The calibration curve showed good linearity.The relative standard deviation of ginsenoside Rg1、Re、Rf、Rb1、Rc、Rb2 and Rd were 2.5%、2.9%、3.8%、1.5%、3.6%、1.9% and 2.3%.The recovery were 102.6%、99.6%、98.7%、101.3%、99.2%、98.5% and 101.8%.It has the advantage of simplicity,fast determination and less reagent consumed in determination of ginsenoside Rg1、Re、Rf、Rb1、Rc、Rb2 and Rd in Abstract of panax ginseng.