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小尾寒羊BMPR-IB基因编码区(CDS)克隆及真核表达载体的构建

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摘要 用Trizol法从羊的卵巢中提取总RNA,反转录成cDNA,用带有酶切位点的BPR-IB基因特异性引物扩增BPR-IB基因完整编码区序列,连接到T载体、测序,序列无误后亚克隆入真核表达载体pEGFP-N2中,进行酶切及PCR鉴定。酶切及PCR鉴定表明成功构建了真核表达载体pEGFP-N2-BMPR-IB。
作者 孙洪新 王红娜 张英杰 刘月琴 李雪梅 李兰会
机构地区 河北省畜牧兽医研究所 河北农业大学动物科技学院
出处 《中国草食动物科学》 CAS 2014年第S1期88-90,共3页 China Herbivore Science
基金 国家肉羊产业技术体系(CARS-39)
关键词 小尾寒羊 BMPR-IB基因 真核表达载体
分类号 Q78 [生物学—分子生物学] R346 [医药卫生—基础医学]
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中国草食动物科学
2014年 第S1期

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