茶树根系Actin基因克隆及表达分析

Gene Cloning and Expression Analysis of Actin in Tea Plant Root

采用SSH技术分析了VA菌根处理后福鼎大白茶根系基因差异表达情况,获得了差异序列,序列比对显示,在下调表达序列中可能包含了10种未知功能的基因;在上调表达序列中可能包含了5种可能的基因。采用RACE技术获得了Actin基因全长序列,Actin基因长1 606 bp(Gen Bank,登录号KJ946252),具有1 131bp开放阅读框(1st^1 131st),编码377个氨基酸。分子生物信息学分析表明,Actin蛋白分子量约30.69 k D,等电点为5.27,定位于细胞核等亚细胞区位。研究还显示,Actin在不同品种中表达无显著差异,对非生物性胁迫响应也较弱。

By using SSH, we analyzed differences in gene expression of root from Fuding white tea infected by VA mycorrhiza and obtained diversity sequences. The sequence alighment showed that the down-regulated expression sequence possibly contained 10 unknown genes and the up-regulated expression sequence possible contained 5 known genes. The Actin genic full-length sequence was obtained by using RACE. The length of Actin gene was 1 606 bp(Gen Bank Accession No. KJ946252), with 1 131 bp ORF(1st-1 131st), the sequence encoded 377 amino acid. Bioinformatics indicated that the Actin protein's molecular weight was about 30.69 k D, IEP was 5.27, located in subcellular fraction area like cell nucleus. The study also showed Actin expressed no obvious difference in different cultivars and it responded weak to non-biological stress.