基于柱花草DUS测试性状的多样性分析与测试指南修订探讨

Diversity of Stylosanthes by using DUS testing and revision of testing guidelines

为了完善柱花草(Stylosanthes)DUS测试体系,本研究采用《植物新品种特异性、一致性和稳定性测试指南柱花草》报批稿中的24个必测性状对192份柱花草种质进行测试,聚类分析得到相似系数为0.770时可将其分为11类,同时结合种质的来源、选育背景及多样性分析的结果,对测试性状中6个必测性状描述补充,剔除荚果喙性状和新增茎的颜色、托叶毛况这两个性状,另外确定了测试标准品种。柱花草DUS测试指南的修订,旨在为柱花草新品种审定及品种鉴定提供更有力的依据。

In order to improve the DUS testing system,through the Guidelines for the Conduct of Tests for Distinctness,Uniformity and Stability Stylosanthes,192 germplasm of Stylosanthes were tested by 24 measured traits. We transfermed test results as code to conduct cluster analysis. And cluster analysis showed that 192 germplasm could be divided into 11 class when the similarity coefficient was 0. 770. Combined with the source of the germplasm,breeding background and results of diversity analysis,explanation of six testing characteristics were to be supplement,the pod beak was to be rejected,the color of coleoptile and the wool of stipule were to be increased. And the test standard varieties were determined. Explored the revision of the guidelines for the conduct of tests for distinctness,uniformity and stability Stylosanthes,can provide more powerful basis for the examination and approval of new varieties and species identification in Stylosanthes.