转GsbZIP33基因苜蓿的耐盐性分析

Salt stress tolerance of transgenic alfalfa with the GsbZIP33 gene

本研究以前期获得的耐盐转基因苜蓿(Medicago sativa)的两个株系b32和b77及其受体对照野生型苜蓿品种为材料,经200mmol·L-1 NaCl处理15d后,系统地测定了与耐盐性相关的生理生化指标,包括叶片净光合速率(Pn),抗氧化酶(SOD,CAT,POD)活性、可溶性糖含量,Na+、K+含量等。结果表明,与野生型相比,转基因苜蓿新株系b32、b77的可溶性糖含量分别增加了48.8%和39.6%;SOD活性分别升高了71%和89%;CAT活性分别升高了21%和13%;POD活性分别升高了32.9%和34.1%,Na+含量分别降低了57%和44%,K+含量分别增长了21%和13%;两个转基因株系(b32、b77)的光合速率是对照的2.4倍和2.5倍,以上指标差异均极显著(P<0.01),表明转GsbZIP33的两个株系b32和b77提高了耐盐性。

In the present study,the physiological and biochemical indicators of two salt-tolerant transgenic lines(b32and b77)and the wild type alfalfa were compared after 15 days treated with 200 mmol·L-1NaCl,including soluble sugar content,the activities of catalase(CAT),peroxidase(POD)and superoxide dismutase(SOD),photosynthetic rate(Pn)and the content of K+and Na+.Compared with the wild type plants,the soluble sugar content of the two transgenic lines increased by 48.8%and 39.6%,respectively,the SOD activities increased by 71% and 89 %,CAT activities increased by 21%in b32 and 13%in b77,POD activities increased by 32.9%and 34.1%,Na+contents decreased by 57%and 44%and K+contents increased by 23% and 13%,respectively.The photosynthetic rate of transgenic plants increased by 2.4times compared with the wild type plants.This study demonstrated that the transgenic alfalfa(b32and b77)had a greater tolerance of saline stress compared with non-transgenic plants.