丹参和川芎嗪对庆大霉素肾毒性的保护作用

Protective effects of Salvia miltiorrhiza and ligustrazine on the damage of rat kidney induced by gentamicin

目的 研究中药丹参、川穹嗪防治庆大霉素引起的肾毒性。方法 给大鼠腹腔注射庆大霉素制成中毒模型,分别注射丹参、川芎嗪预防肾毒性,用药前、后检测大鼠的肾功能、尿酶等指标,并进行肾组织光镜、电镜检查。结果 发现各组尿NAG酶均比正常组增高,其中模型组明显增高。各组血清中超氧化物歧化酶(SOD)活性均比正常组降低,而模型组下降更明显,血中的尿素氮及肌酐含量均比正常组增高,模型组增高更明显,形态学检查可见模型组肾近曲小管上皮细胞广泛性坏死,各预防组可见片状变性。结论 丹参、川芎嗪对庆大霉素的肾毒性有预防作用。

Objective To study the effects of Sal via miltiorrhiza and ligustrazine on the nephrotoxicity caused by gentamicin. Methods Rats were divided into 1 .normal group, 2. toxic model group, 3. Salvia miltiorrhiza group, 4.ligustrazine treated group. The toxic model was made by intraperitoneal injection of gentamicin. Renal function, vorkinase and morphological changes were detected at different internals. Results Urine NAG and serum Scr、BUN were significantly higher in group 2 - 4 than that in group 1 and group 2 much higher than other groups. Serum SOD activity decreased singnificantly in 2 - 4 groups than normal group and in model group it decreased more prominently. Light microscopy showed extensive degeneration and necrosis in proximal tubular epithelial cells and vacuolization in distal tubules in model group but only focal to patch degenration in ligustrazine and Salvia miltiorrhiza groups and necrosis was seldom. Conclusion Salvia miltiorrhiza and ligustrazine may play a role in the protection of nephrotoxicity induced by gentamicin.