血小板生成素过量表达对T淋巴细胞增殖和分化的影响

Thrombopoietin Over expression Stimulates T lymphocyte Proliferation and Differentiation.

目的 探讨ＴＰＯ（血小板生成素）基因导入对Ｔ 细胞增殖和分化影响的规律。 方法 将编码人ＴＰＯ 的质粒６０μｇ 用脂质体包裹后，经肌肉途径转导入Ｂａｌｂ／ｃ 小鼠体内。用ＣＤ４和ＣＤ８的特异性抗体标记小鼠各种免疫组织中的Ｔ 细胞，借助流式细胞术分析它们的组成和变化。 结果 发现ＴＰＯ 基因导入使（１）骨髓中的成熟和未成熟Ｔ 细胞数量在经过短期下降后全部大幅度增多；（２）胸腺未成熟Ｔ细胞增加；（３）脾脏和血液循环中成熟Ｔ 细胞增多，特别是在血液中ＣＤ４阳性细胞的增加最为明显。这些特点大约在基因导入后两周内形成，并持续至少两周以上。 结论 提示ＴＰＯ 是一种免疫促进分子，ＴＰＯ 基因导入后小鼠血液中多种淋巴因子的升高与它过量表达对Ｔ 细胞的刺激有关。

To elucidate the regular effect pattern of thrombopoietin (TPO) on the differentiation and proliferation of T lymphocytes via gene transference. Methods 60μg human TPO coding pcDNA3 plasmid coated with lipofectin (10μg/μl) was delivered into each Balb/c mice. Monoclonal antibodies against murine CD4 or CD8 molecules were employed to directly stain the mononuclear cells from the immune tissues, they were analysed by flow cytometry. Results Mice treated with null plasmid or saline were made as controls. It was discovered that with the delivery of TPO cDNA, both mature and the immature T lymphocytes in bone marrow increased in frequencies after a transient declination. Immature T lymphocytes increased in thymuses, while mainly mature T lymphocytes increased in spleens and blood circulation. Significantly among them was that CD4+ T subsets selectively proliferated in the process. They had formed the characters around 2 weeks after gene transference and remained such ratios for at least 2 weeks. Conclusion TPO be an immunoregulator and the cytokine over production in gene therpay mice is due to the activation of T lymphocytes, especially the helper subgroups.