白色念珠菌酮康唑抗药株的筛选

Screening of ketoconazole resistant strains from Candida albicans

目的获得白色念珠菌的酮康唑抗药株。方法采用美国国家临床实验标准化委员会 (NC CLS)推荐的最小抑菌浓度 (MIC)测定法 ,从 8株不同来源的白色念珠菌中筛选出YS2 0 1 (MIC为0 0 62 μg/mL)作为出发菌株 ,分别利用紫外线 (UV)和亚硝基胍 (NTG)对其原生质体进行诱变。结果经大量筛选 ,得到抗药突变株UP1 1及NP2 9,其MIC分别达到 3 5 μg/mL和 75 μg/mL。结论获得酮康唑抗药株 ,突变株的抗药特性稳定。

Objective To obtain the ketoconazole resistant strains. Methods We adopted the method of the minimum inhibitory concentration (MIC) assay recommended by National Committee for Clinical Laboratory Standard (NCCLS) to screen the original strains. YS201(MIC 0 0625 μg/mL ) was used as the original strain by screening from the 8 strains of Candida albicans which have different origins. Results We finally obtained the ketoconazole resistant strains UP11 and NP29 with their MIC up to 35 μg/mL and 75 μg/mL with using UV and NTG mutagenesis to Candida albicans protoplasts respectively. Conclusions Ketoconazole resistant strains were obtained and the subculture test showed that the drug resistant characteristics of the strains are stable.