聚磷酸钙纤维体内降解及组织相容性实验

EXPERIMENT OF HISTOCOMPATIBILITY AND DEGRADATION IN VIVO OF ARTIFICIAL MATERIAL CALCIUM POLYPHOSPHATE FIBER

目的 研究人工合成聚磷酸钙纤维体内降解及组织相容性 ,为肌腱组织工程材料研究提供部分实验依据。方法 聚磷酸钙纤维束和对照材料碳纤维在 2 0只 SD大鼠肢体对称部位的皮下、肌肉、肌腱内植入。术后 1个月观察实验鼠的日常活动 ;术后当日 ,4、8、12、16及 2 0周行 X线片检查 ;术后 4、8、12、16及 2 0周取出材料 ,行肉眼观察、组织切片 ,HE染色。结果 X线片及组织切片证实聚磷酸钙纤维在肌肉内约 16周完全降解 ,肌腱、皮下约 2 0周完全降解 ,降解产物无局部沉积现象 ;而对照碳纤维在实验中未观察到明显降解。聚磷酸钙纤维周围组织内淋巴细胞及巨噬细胞明显少于碳纤维周围 ,且与周围增生组织相融合程度亦优于碳纤维。结论 聚磷酸钙纤维在体内 16～ 2 0周可完全降解 。

Objective To study the histocompatibility and degradation in vivo of a new artificial material, calcium polyphosphate fiber (CPPF), and to provide some experimental basis for further study in tendon tissue engineering. Methods CPPF and carbon fiber (CF) as control material were implanted in symmetry part in subcutaneous layer, muscle and Achilles tendon of 20 SD rats. The day of operation, the 4th, 8th, 12th, 16th, and 20th weeks after operation, X ray examination was performed to detect the density change of materials. Local tissue and materials were observed grossly, and pathological examination was made with HE staining 4, 8, 12, 16 weeks after operation. Results CPPF degraded completely within 16 weeks in muscle, and in 20 weeks after implantation in Achilles tendon and subcutaneous layer according to X ray and pathological examination. No calcium phosphate crystal deposit was observed in local tissue. No obviously degradation of CF was found within 20 weeks. Local infiltration of lymphocytes and macrophagocytes around CPPF were much fewer than that of CF. CPPF combined compactly with surrounding hyperplastic tissue. Conclusion CPPF degrade thoroughly from 16 to 20 weeks without sediment of crystal of calcium phosphate in vivo. CPPF has good histocompatibility and can be used as a scaffold material of tissue engineering.