复合可降解基质膜片制备及血管内皮细胞在其吸附生长的实验研究

PREPARATION OF COMPOUND BIODEGRADABLE MATRICES AND GROWTH OF VASCULAR ENDOTHELIAL CELL ON THEM

目的 制备几种复合可降解基质膜片 ,观察血管内皮细胞在其上的生长情况。方法 用胶原酶消化法分离牛主动脉血管内皮细胞 (VEC) ,另采用两步盐析法提取牛骨 型胶原。将交联胶原包埋处理聚羟基乙酸 (PGA)、聚乳酸 (PL A)及聚β羟基丁酸 (PHB)形成可降解基质材料膜片 ,并接种牛 VEC,采用 MTT法比较 VEC在几种可降解基质材料膜片的生长情况。结果 胶原、PGA/胶原、PL A/胶原膜质地均匀柔韧 ,固定成形 ,具有一定弹性和吸水性 ;MTT比色实验表明 VEC在胶原、PGA/胶原、PL A/胶原上均生长良好 ,优于 PHB/胶原组。尤以 PGA/胶原所形成的基质材料固定成形 ,弹性和韧性好 ,VEC在其上贴附生长良好。结论 通过生物材料与人工可降解材料有机结合 ,PGA/胶原物理性能互补 ,是组织工程再造血管较理想的基质材料之一。

Objective To prepare the compound biodegradable matrices, polyglycolic acid (PGA), polylactic acid (PLA) mesh and poly β hydroxybutyrate(PHB) which precoated with collagen, and to observe the growth and differentiation of bovine vascular endothelial cells on these scaffolds. Methods By enzymatic digestion methods, bovine vascular endothelial cell (VEC) were isolated from calf thoracic aorta, then cultured and purified. PGA, PLA, PHB meshes were dipped into cross linked type I collagen solution, dried under vacuum frozen condition. VEC were seeded into these scaffolds. The growth of VEC on scaffolds was analyzed by MTT method. Results The collagen, PGA/collagen, PLA/collagen scaffolds were elasticity and tenacity. VEC grew better on collagen, PGA/collagen, and PLA/ collagen membranes than on the PHB/collagen one. Conclusion The PGA/collagen scaffold has elasticity, plasticity and tenacity. VEC grow best on it. It is an ideal scaffold for tissue engineered vessel reconstruction for it integrating both advantages of biomaterials and degradable materials.