碱性成纤维细胞生长因子和硫糖铝联合局部应用对扩张皮肤组织结构的影响

EFFECT OF LOCAL APPLICATION OF BASIC FIBROBLAST GROWTH FACTOR AND SUCRALFATE ON SKIN TISSUE STRUCTURE AFTER EXPANSION

目的 探讨碱性成纤维细胞生长因子 (b FGF)和硫糖铝对扩张皮肤组织结构的影响。方法 白色小猪 9只 ,自身对照。在猪两侧侧胸埋置皮肤扩张器。持续恒压扩张同时实验 组注入 b FGF和硫糖铝 ,实验 组注入b FGF和生理盐水 ,实验 组注入硫糖铝 ,对照组注入生理盐水共 7天。各组完成扩张后第 3天及取出扩张器后 6周取材行组织学检查。结果 实验 组皮肤表皮层增厚更明显 ,颗粒和棘细胞层次更多 ,基底细胞数目增多 ,细胞核肥大 ,染色质变浓 ,排列密集 ,表皮钉突增多 ,呈粗长发育成巨大钉突 ;真皮层厚度变化较轻 ,胶原纤维束粗而密 ,平行于皮面排列 ,弹力纤维明显增加 ;成纤维细胞和毛细血管密度更高 (P<0 .0 5 )。对照组表皮层和真皮层有相应的变化均弱于实验 组 ,术后 3天、6周胶原纤维可见断裂。实验 、 组表皮层和真皮层组织改变与对照组差异无统计学意义 (P>0 .0 5 )。各组纤维包膜厚度近似。结论 在持续恒压扩张同时扩张囊周围注入外源性 b FGF和硫糖铝 ,能更有效地刺激皮肤生物性生长 ,促进扩张速率 。

Objective To investigate the effect of local application of basic fibroblast growth factor (bFGF) and sucralfate on the skin tissue structure after expansion. Methods Continuous tissue expansion (CTE) experimental animal model was made with constant pressure pump in nine white pigs. The bFGF and sucralfate were perfused between tissue and tissue expander. Both bFGF and sucralfate were injected in group I, both bFGF and normal saline in group II, only sucralfate in group III, and normal saline in group IV as control group. The samples were took from each pig for histomorphological assessment on the 3rd day and 6th week after expansion. Results Compared with control group, epidermal, granular spinous and basal cell thickness increased significantly ( P< 0.05) in group I; in dermal layer, dermal thickened slightly and the collagen fibers became thicker and orderly; the elastic fiber regenerated significantly; the density of fibroblasts and capillaries significantly increased ( P< 0.05) in group I. The collagen fibers were broken on the 3rd day and 6th week after expansion in control group. However, the thickness of the fibrous capsule layer was similar in each group. Conclusion Local application of exogenous bFGF and sucralfate combined with CTE can greatly promote the skin growth. It can accelerate the tissue expansion.