版纳微型猪近交系椎间盘异种抗原α-半乳糖基的分布

EXPRESSION AND DISTRIBUTION OF XENOANTIGEN α-GAL IN INTERVERTEBRAL DISK OF CHINESE BANNA MINIPIG INBRED LINE

目的 探讨中国版纳微型猪近交系 (BMI)椎间盘组织中异种抗原的分布 ,为临床猪 -人异种椎间盘移植提供依据。方法 采用 BMI 6头 ,雄性 ,8～ 11月龄的 L1～ 5椎间盘组织 ,常规福尔马林固定 ,石蜡包埋切片。以植物凝集素 (BSI- B4 )作为亲和物质 ,采用免疫组织化学 SABC方法、DAB显色 ,观察猪椎间盘组织中异种抗原 (α-半乳糖基 )的分布情况。结果 椎间盘纤维环软骨细胞及髓核软骨样细胞核膜有阳性染色的黄色颗粒沉着 ,而基质、弹力及胶原纤维无着色。结论 BMI椎间盘组织中有异种抗原存在 ,但抗原表达较弱 。

Objective To investigate the expression and distribution of xenoantigen in intervertebral disk of Chinese banna minipig inbred line, and to study the availability of xenograft transplantation of intervertebral disk. Methods Samples of intervertebral disk were collected from six Banna pigs of 8 to 11 month old. The fixation, embedment and slice were performed. α Gal specific binding lection (BSI B 4) were used as affinity reagents and affinity immunohistochemistry assays (SABC methods and DAB stain) were conducted to detect the expression and distribution of xenoantigen (α Gal). Results α Gal was found in chondrocyte cell and chondrocyte like cell in intervertebral disk which have the positive yellow stained particulate aggradation. There was no stain in the matrix, elastic fiber and collagen fiber. Conclusions The distribution of xenoantigen is locally in the tissue of intervertebral disk and its expression is weak. This suggests that the intervertebral disk of Banna pig may be alternative donor for xenotransplantation.