复方861含药血清对HSC-T6细胞Ⅰ型胶原、Ⅲ型胶原mRNA表达影响的研究

Effect of Compound 861-contained Serum on CoL-Ⅰ,CoL-Ⅲ Gene Expression of HSC-T6 Cells

观察复方 86 1含药血清对HSC T6细胞Ⅰ型胶原 (CoL Ⅰ )、Ⅲ型胶原 (CoL Ⅲ )mRNA表达影响。以不同剂量复方 86 1灌胃大鼠制备的含药血清 ,作用HSC T6细胞 4 8h ,应用逆转录定量PCR方法测定其对HSC T6细胞CoL Ⅰ、CoL ⅢmRNA表达的影响。结果表明 :复方 86 10 5、1.0、2 .0、6 .0g kg等不同剂量含药血清HSC T6细胞CoL Ⅰ、CoL ⅢmRNA表达。与空白对照组比较 ,具有显著性 (P <0 .0 5或P ,0 .0 1)。因此 ,复方 86 1可降低HSC T6细胞CoL Ⅰ、CoL ⅢmRNA表达水平 ,具有抗肝纤维化作用。

To observe the effect of Compound 861 contained(Cpd 861) serum on CoL Ⅰ、CoL Ⅲ mRNA expression of HSC T6 cells in vitro,HSC T6 cells were exposed in different doses of Cpd 861 contained serum (0.5,1.0,2.0,6.0g/kg) for 48 hours.CoL Ⅰ、CoL Ⅲ mRNA levels were measured by quantitative reverse transcription polymerase chain reaction (RT PCR).CoL Ⅰ、CoL Ⅲ mRNA levels of HSC T6 cells at different doses of Cpd861 contained serum were lower than those of normal control.Cpd 861 contained serum can decrease CoL Ⅰ and CoL Ⅲ mRNA expression of HSC T6 cells.