复发性口腔溃疡患者血清中微量元素含量及细胞免疫功能的研究

Studies on the Content of Trace Element in Serum and the Condition of Cell-Mediated Immunity in Patients with Recurrent Aphthous Ulceration

目的 :探讨复发性口腔溃疡 (RAU)患者血清中微量元素含量及细胞免疫功能状态。方法 :采用原子吸收分光光度计检测血清中微量元素的含量 ,流式细胞仪检测外周血 T细胞亚群和 NK细胞百分比 ,ELISA法检测血清中细胞因子。结果 :RAU组血清中锌和硒的含量均明显低于对照组 (均 P<0 .0 1 ) ,而镁、铁、铜的含量与对照组之间的差别无显著性的意义 (均P>0 .0 5 ) ;RAU组外周血中 CD3 + 、CD4 + 、CD4 + /CD8+ 和 CD5 6 + 细胞 (NK细胞 )的比例均明显低于对照组 (均 P<0 .0 1 ) ,RAU组血清中 IL- 1 2产生水平明显低于对照组 (P<0 .0 1 ) ,而 IL- 1 0产生水平明显高于对照组 (P<0 .0 1 )。结论 :RAU患者存在细胞免疫功能缺陷 ,临床治疗须提高 RAU患者的细胞免疫功能 ,合理补锌和补硒对于防治

Objective: To investigate the content of trace element in serum and the condition of cell-mediated immunity in patients with recurrent aphthous ulceration (RAU). Methods: Atomic absorption spectrometer was used to monitor the content of trace element in serum. Flow cytometer was used to test T-cell subpopulation and NK cell in peripheral blood. ELISA method was used to examine cytokine in serum. Results: The contents of Zn and Se in serum of RAU patients were obviously lower than those of contrastive group (P<0.01),while the contents of Mg, Fe, Cu of RAU patients and contrastive group did not have obvious difference(P> 0.05). The RAU patients showed lower levels of CD 3 + T-cell,CD 4 + T-cell,CD 4 +/CD 8 + T-cell ratio, CD56 +T-cell(NK cell) and IL-12 than those of the contrastive group(P<0.01),while the level of IL-10 of the RAU was higher(P<0.01). Conclusion: Cellular immunodeficiency exists in RAU patients, so cellular immune function should be improved during clinical therapy. Furthermore, supplementing Zn and Se properly is useful to prevent and cure RAU.