摘要
目的 :探讨复发性口腔溃疡 (RAU)患者血清中微量元素含量及细胞免疫功能状态。方法 :采用原子吸收分光光度计检测血清中微量元素的含量 ,流式细胞仪检测外周血 T细胞亚群和 NK细胞百分比 ,ELISA法检测血清中细胞因子。结果 :RAU组血清中锌和硒的含量均明显低于对照组 (均 P<0 .0 1 ) ,而镁、铁、铜的含量与对照组之间的差别无显著性的意义 (均P>0 .0 5 ) ;RAU组外周血中 CD3 + 、CD4 + 、CD4 + /CD8+ 和 CD5 6 + 细胞 (NK细胞 )的比例均明显低于对照组 (均 P<0 .0 1 ) ,RAU组血清中 IL- 1 2产生水平明显低于对照组 (P<0 .0 1 ) ,而 IL- 1 0产生水平明显高于对照组 (P<0 .0 1 )。结论 :RAU患者存在细胞免疫功能缺陷 ,临床治疗须提高 RAU患者的细胞免疫功能 ,合理补锌和补硒对于防治
Objective: To investigate the content of trace element in serum and the condition of cell-mediated immunity in patients with recurrent aphthous ulceration (RAU). Methods: Atomic absorption spectrometer was used to monitor the content of trace element in serum. Flow cytometer was used to test T-cell subpopulation and NK cell in peripheral blood. ELISA method was used to examine cytokine in serum. Results: The contents of Zn and Se in serum of RAU patients were obviously lower than those of contrastive group (P<0.01),while the contents of Mg, Fe, Cu of RAU patients and contrastive group did not have obvious difference(P> 0.05). The RAU patients showed lower levels of CD 3 + T-cell,CD 4 + T-cell,CD 4 +/CD 8 + T-cell ratio, CD56 +T-cell(NK cell) and IL-12 than those of the contrastive group(P<0.01),while the level of IL-10 of the RAU was higher(P<0.01). Conclusion: Cellular immunodeficiency exists in RAU patients, so cellular immune function should be improved during clinical therapy. Furthermore, supplementing Zn and Se properly is useful to prevent and cure RAU.
出处
《微量元素与健康研究》
CAS
2002年第3期16-18,共3页
Studies of Trace Elements and Health