摘要
目的 :建立内服液体制剂中羟苯甲酯和羟苯乙酯的反相高效液相色谱测定方法。方法 :色谱柱 :Nova -ParkC18 柱(3 9mm×150mm ,5μm) ;流动相 :乙腈 -水 (25∶75) ;流速 :1ml/min ;检测波长 :254nm ;灵敏度 :0 01AUFS。结果 :羟苯甲酯浓度在0 4412~13 2360μg/ml范围内线性关系良好 (r=0 9999) ;羟苯乙酯浓度在0 6672~20 0160μg/ml范围内线性关系良好(r=0 9998)。结论 :该方法简便、快速、准确 ,可用于医院制剂中防腐剂的检测。
OBJECTIVE:To establish the RP-HPLC method for determination of methyl hydroxybenzoate and ethyl hydroxybenzoate in oral liquid preparations METHODS:The Nova-Park C18 column(3 9mm×150mm,5μm)was used The mobile phase consisted of acetonitrile-water(25∶75)at a flow rate of 1ml/min,the detection wavelength was set at 254nm and the sensitivity was set at 0 01 AUFS RESULTS:The linear ranges of methyl hydroxybenzoate and ethyl hydroxybenzoate were 0 4 412~13 2 360μg/ml(r=0 9 999) and 0 6 672~20 0 160μg/ml(r=0 9 998)respectively CONCLUSION:The method is simple,rapid and accurate It can be used to detect antiseptics in hospital preparations
出处
《中国药房》
CAS
CSCD
2002年第9期553-554,共2页
China Pharmacy