三唑酮对离体黄瓜子叶过氧化物酶活性及蛋白质含量的影响

Effect of Triadimefon on Activity of Peroxidase and Soluble Protein Content in Detached Cucumber Cotyledons

20mg/L三唑酮能显著地抑制离体黄瓜子叶叶绿素a、b、可溶性蛋白质和游离脯氨酸含量及叶绿素a/b的下降 ,表明三唑酮抑制子叶的衰老 .与此同时 ,三唑酮能不同程度地抑制过氧化物酶 (POD)活性升高和H2 O2 的累积 .在黄瓜子叶衰老过程中 ,叶绿素及蛋白质含量均与POD、H2 O2 成显著的负相关 ,表明三唑酮可能通过POD H2 O2

Contents of chlorophyll a, chlorophyll b, soluble protein and proline, as well as the ratio chlorophyll a/b in detached cotyledons of cucumber were distinctly increased by treating with 20 mg/L triadimefon, which suggested that triadimefon can effectively retard the senescence of detached cotyledons in cucumber. Triadimefon could inhibit the increase of peroxide (POD) activity and H 2O 2 content in different degree. It was found that contents of chlorophyll and protein were markedly negative correlated with the activity of POD and with the content of H 2O 2 respectively during the senescence of cotyledons. These results indicated triadimefon inhibit the degradation of chlorophyll and protein in cucumber cotyledons through POD H 2O 2 enzyme system.