同步荧光法检测芘的微生物降解

Detection of biodegradation of pyrene by synchronous fluorometry.

采用同步荧光法研究矿物盐介质中溶解态芘的生物降解速率.所建方法对溶解态芘的检测限为0.19ng/mL,相对标准偏差小于1.3%(n=9).在相同的实验条件下,用所建方法考察了3种不同芘的降解菌株对溶解态芘的降解能力.结果表明,该方法最大的特点是在不经萃取的情况下可直接用于溶解态芘的降解过程的检测,所得结果与GC/FID的实验结果一致.与现行的GC法相比较,该方法每一次检测的时间小于1min,仪器及运行成本很低.如果能与现行的研究方法相结合,该方法的建立可为在实验室或现场研究PAHs的生物降解及衰减提供一有力的研究手段.

The synchronous fluorometry has been adopted to study the biodegradation rate of pyrene dissolved in mineralsalts media (MSM). The limit of detection is calculated as 0.19ng/mL for the dissolved pyrene with the relative standard deviation less than 1.3% (n=9). The capability of three kinds of pyrene degrading strains has been investigated by the established method under same experimental condition. The results show that greatest feature of the process is its capability to detect directly without extraction. The result obtained is identical with the result of GC/FID experimentCompared with the GC methods used currently, this method has its detecting time less than 1 min, and the cost ofinstruments and operation is quite low. If the established method are combined with other methods, powerful tools might be obtained to study the biodegradation and the fate of PAHs in laboratory or in field.