蛋白质光谱探针研究进展

Development of Spectroscopic Probes of Protein

蛋白质分析是生命科学研究中的重要课题 .蛋白质内源紫外吸收光谱和荧光光谱可以用于蛋白质分析 ,但其灵敏度不能满足微量分析的要求 .蛋白质光谱探针 (包括金属离子、有机试剂和金属络合物 )能够与蛋白质相互作用生成超分子复合物并引起体系光谱信号的变化 ,由此可以提供蛋白质含量或结构方面的信息 .根据光分析方法的不同 ,蛋白质光谱探针可以分为吸光探针、荧光探针和光散射探针 3类 .本文中 ,笔者综述了蛋白质光谱探针的研究进展 。

Protein analysis is an important task in the study of life science.Inherent ultraviolet absorption spectrum and fluorescence spectrum of protein can be used in protein analysis,but the sensitivity of these methods can not meet the demand of micro determination.Protein spectroscopic probes,including metal ions,organic reagents and metal complex,interact with protein to form supramolecular complex causing spectral change of the system and thereby giving quantitative or constructive information about the protein.According to the photoanalytical method used,protein spectroscopic probes may be divided into three kinds:light absorption probes,fluorescent probes and light scattering probes.