蜂毒溶血肽(Melittin)cDNA的克隆及序列分析

The Cloning and Sequencing of cDNA Encoded Melittin

为了克隆得到正确序列的蜂毒溶血肽 c DNA,对蜂毒中的蜂毒溶血肽进行了研究。实验从蜜蜂毒腺中提取总 RNA,通过 RT- PCR方法扩增得到了蜂毒溶血肽的 c DNA,扩增产物克隆到p T7Blu- T载体 ,转化大肠杆菌 JM1 0 9,DNA序列分析结果表明克隆得到的蜂毒溶血肽 c

The melittin in bee poison gland was studied to obtain the correct cDNA encoded melittin. During the experiment, the total RNA was obtained from bee poison gland and cDNA en\|coded melittin was obtained by RT PCR. After that, the obtained product was ligated to pT7Blu T vector and the recombined plasmid was transformed to the E. coli strain of JM109. The result of DNA sequence analysis demonstrated that the sequence of cDNA encoded melittin was correct.