重离子射线照射对家蚕造血器官的生理效应

Physiological Effect of Heavy Ion Beam Irradiation on Larval Hemopoietic Organs of Silkworm,Bombyx mori

用碳离子和氦离子射线局部照射家蚕 5龄第 3日幼虫的造血器官 ,调查了对循环血球密度和种类 ,以及其后生长发育和变态的影响。照射后家蚕变态困难 ,结茧率、化蛹率和羽化率显著降低 ;幼虫血液中血球密度在照射后 8～ 2 4h短暂上升 ,随后快速下降 ,5龄后期血球密度显著低下。上述变化程度随射线剂量增加而更加显著 ,而两种离子射线之间没有明显差异。血液中各种血球的比例虽然没有很大变化 ,但照射后出现了一种大型血球。

The thoracic regions,where the imagine wing discs are located and to which the hemopoietic organs are attached,of third day silkworm larvae in fifth instar were locally irradiated with ion beam ( 12 C 5+ and 4He 2+ ).The hemocyte density and hemocyte type in larva,and development and metamorphosis of larvae and pupae were observed.The irradiated silkworms showed difficulty in their metamorphosis,and to be markedly lowered in cocooning rate,pupation rate and eclosion rate.Compared to the control,the hemocyte density in irradiated silkworms were found to be significantly higher during 8～24 hours after irradiation,then decreased quickly and to be significantly low during the late stages of 5th instar.With the dose of irradiation ions beam being increased,the adove mentioned changes could become strong.There were no marked difference between the effects of 12 C 5+ and 4He 2+ on above mentioned phenomena.A big type of hemocyte was found in irradiated larvae,although the established types of hemocyte and their ratio had not changed.