摘要
从含有鹅细小病毒 (GPV)H1株主要结构蛋白VP3基因的重组质粒pPROEXHTb VP3中切取GPVH 1株VP3基因片段 ,将其亚克隆于 pSY5 3 8的EcoRⅠ位点 ,并将带有痘苗病毒启动子P11的LacZ报告基因平端克隆于上述重组子的SmaⅠ位点 ,再用NotⅠ切下同时含有VP3基因和LacZ报告基因的片段 ,亚克隆于pSY681的NotⅠ位点 ,构建了含有VP3基因的重组禽痘病毒转移载体。
VP3 gene of H1 isolate of goose parvovirus derived from recombinant plasmid pPROEX HTb VP3 was subcloned into EcoRⅠ site of pSY538, and the reporter gene LacZ with promoter p11 was cloned into SmaⅠ site of this plasmid .Both VP3 gene and LacZ gene were cloned into NotⅠ site of pSY681,recombinant FPVtransfer vector containing VP3 gene of GPV was obtained.The result is basis of development of GPV genetic engineering vaccine.
出处
《中国兽医科技》
CSCD
北大核心
2002年第9期5-8,共4页
Chinese Journal of Veterinary Science and Technology
基金
黑龙江省"十五"攻关项目 (GB0 1B50 3 0 2 )
