摘要
本文通过对Ｌｏｗｉｃｒｙｌ Ｋ４ Ｍ 的使用方法上的改进，成功地将其运用于大豆免疫电镜研究中，并得到良好的效果；通过对各种胶囊紫外吸收光谱分析及实际比较应用，对Ｋ４ Ｍ 在大豆免疫电镜中的应用条件进行了细致的探讨，发现以４ ％ 多聚甲醛和２ ．５ ％ 戊二醛固定材料１ 小时，室温渗透并逐渐降低温度，用国产白色胶囊包装Ｋ４ Ｍ ，在－ １４ ℃中１０ｃｍ 距离下紫外光照射４ 小时，在３０ ———４０ｃｍ 的ＵＶ 照射下４８ 小时，室温固化２ —３ 天，均可以得到较好的聚合效果。
This paper reports that Lowieryl K4M resin was succssful in applying it for the research of Soybeam immune electron microscopy (IEM),Through improving the usage of the resin,While has gotten the excellent results;and studied on the conditions of usage seriously for Lowicryl K4M resin in the Soybeam IEM,by the use and comparative test of the ultraviolet(UV) absorption analysis for all sorts of capsules,The results reveal that white gelatin capsules made in China get better than others for loading the K4M resin to UV polymerization at the conditions:fixation in 4% polyformadehyde and decreasing temperature gradually,polymerization at -14℃、distance at 10cm for 24 hours under UV-Light for 48 hours,then transfer them to room temperature for 1-2 days to hardenning,these all may get the good effects on polymerization,Which has some practial value for other plant materials on the immuno-electron microscopy with Lowicryl K4M.
