光纤荧光法在线监测家兔柔红霉素血药浓度

Continuous Monitoring of Blood Daunorubicin with Fiber Optic Fluorometry

目的:探索将光纤传感器作为一种新的获取分析物信息的方法用于体内药物过程监测的可行性。方法:通过光的准确聚焦,使进入直径为100μm的光纤中的光强度增加,能够进行荧光信号的检测;同时设计了简便可行的动物模型,使得光纤探头可以直接插入血管对检测对象进行检测分析;以柔红霉素的特征荧光作为传感器对药物的识别分离手段,进行了柔红霉素在家兔体内过程的监测分析。结果:方法的回收率在规定的浓度下为99.9％～108.0％,日内RSD为2.5％～5.3％,当信噪比为3时,检出限为0.05μg·mL^(-1)。结论:此法简便,为在线在位监测其他生物体液中药物浓度及药物在生物体组织器官中的分布代谢提供了可以借鉴的新方法和新技术。

Objective: To evaluate the feasibility and accuracy of continuous monitoring of drug with fiber optic fluorometry in animal model. Methods: An accurate optical design was used to enhance the intensity of light from a 100 - micron optic fiber and the fluorescence signal can be detected. Daunorubicin (DNR) is determined by fiber optic fluorometry system based on the feature fluorescence spectrum. In a simple animal model, the carotid artery was catheterized with a cannula, housing a 100 - micron optic fiber. The vary quenching indicated the concentration of DNR in rabbit's blood. Results: The average recovery of all the tested compounds within the set concentration range was 99. 9% - 108. 0% , the within - day reproducibility value were acceptable within 2. 5% -5. 3% respectively. The method permitted detection limits as low as 0. 05 μg·mL-1 at a signal - to - noise ratio of 3. Conclusion: The method is potentially useful for monitoring drug blood concentration in biomedical field.