一氧化氮与阻塞性黄疸肾功能障碍关系的实验研究

The experimental study of the relationship between nitric oxide and renal insufficiency in obstructive jaundice

目的 :研究一氧化氮 (NO)与阻塞性黄疸 (OJ)肾功能障碍的关系。方法 :雄性SD大鼠胆总管结扎后随机分成 5d、10d及 15d三组 (B1组、B2组、B3组 ) ,同时建立对应的假手术对照组。观察肾功能的变化 ,同时测定血和肾组织NO水平及一氧化氮合酶 (NOS)活性 ,并用图像分析检测NOS mRNA于肾脏表达的部位和量的变化。结果 :B1、B2及B3各组的血及肾组织NO含量分别是 (43.72± 10 .6 1) μmol/L ,(0 .5 15± 0 .0 82 )μmol/g·pro ;(34.4 4± 9.6 3) μmol/L ,(0 .375± 0 .0 96 ) μmol/g·pro ;(2 7.34± 8.88) μmol/L ,(0 .2 5 1± 0 .0 86 ) μmol/g·pro。血及肾组织NO与内生肌酐清除率 (Ccr)、肾皮质血流 (RCBF)呈正相关。肾组织iNOSmRNA表达增加 ,血和肾组织NOS活性降低。结论 :血和肾组织NO水平下降 ,是导致OJ时肾功能损伤的原因之一 ,体内NO水平的持续下降是由于NOS活性降低而非NOS基因表达减少。

Objective:To evaluate the association between nitric oxide and renal insufficiency in obstructive jaundice.Methods:Male sprague Dawley rats who underwent common bile duct ligation randomized into five day(group B1),ten day(group B2) and fifteen day(group B3),meanwhile each matching sham operation group was made. Renal functions were investigated. The concentrations of NO and the activity of NOS in blood and renal tissue were measured. Image analysis were performed to investigate the site and quantity of the expression of NOS mRNA in the renal tissue.Results:The concentrations of NO in plasma and kidney in groups B1,B2 and B3 were(43.72±10.61)μmol/L,(0.515±0.082)μmol/g·pro;(34.44±9.63)μmol/L,(0.375±0.096)μmol/g·pro;(27.34±8.88)μmol/L,(0.251±0.086)μmol/g·pro,the NO levels positively correlated with Ccr and RCBF. The expressions of iNOS mRNA in renal tissue increased while the NOS activity in plasma and renal reduced.Conclusion:Reduced levels of NO in plasma and renal tissue may be one of the main causes of renal dysfunction in obstructive jaundice. Decreased NO levels resulted from depressed activity of plasma and renal NOS instead of the reduced expressions of NOS mRNA.