前列腺素E_2对大鼠急性胰腺炎IL-10抗TNF-α、IL-1β的免疫干预作用

An experimental study on the effect of immuno-intervention with prostaglandin E_2 on IL-10 against TNF-α and IL-1β in rats with acute necrotizing pancreatitis

目的 :探讨前列腺素E2 (PGE2 )免疫干预对急性胰腺炎大鼠血清抗炎因子IL 10与前炎症因子TNF α及IL 1β的平衡调控作用。 方法 :将SD大鼠 91只随机分 4组 :假手术组 (n =7) ,ANP组 ,PGE2 前干预和PGE2 后干预组 (每组n =2 8) ;后三组又随机分 1h、3h、6h以及 12h时段组 (每组n =7) ,大鼠ANP模型用 5 %牛磺胆酸钠诱导 ,PGE2 前干预组在ANP模型制作前 2h肌肉注射 15 0 μg·kg-1PGE2 ,PGE2 后干预组在ANP模型完成时即刻肌肉注射 15 0 μg·kg-1PGE2 。采用ELISA检测各组各时段大鼠血清的IL 10、TNF α与IL 1β含量。结果 :PGE2 前干预组和后干预组所有时段 (1h、3h、6h以及 12h)的TNF α和IL 1β含量均比ANP组降低 ,差异有显著性 (P <0 .0 0 1) ;PGE2 前干预组 3h时段IL 10值比后干预组相应时段升高 ,而 6h时段IL 10含量比后干预组低 ,差异均有显著性 (P <0 .0 0 1)。本实验结果显示 ,大鼠血清IL 10的上升幅度因PGE2 的给药时间不同而不同 ,TNF α和IL 1β随IL 10上升而下降。ANP组血清IL 10与TNF α呈正相关 (r=0 .5 85 ,P <0 .0 1) ;PGE2 前、后干预组呈负相关 (r=- 0 .0 4 5 ,P <0 .0 1;r =- 0 .14 3,P <0 .0 1)。结论 :无论是前干预还是后干预 ,PGE2 均能上调大鼠血清抗炎症性因子IL 10 。

Objective:To study the effect of immuno intervention with prostaglandin E 2 on interleukin 10 against tumor necrosis factor and IL 1β in rats with acute necrotizing pancreatitis.Methods:91 SD rats were randomly divided into 4 groups:sham operation group(n=7);ANP group,pre intervention group and post intervention group(n=28,respectively),which were randomly sub divided into 4 different time groups(1 h,3 h,6 h,12 h,n=7,respectively). Rats of ANP model were induced by retrograde injection of 5% sodium taurocholate into the pancreatic duct. Pre intervention group was intramuscularly injected by 150 μg·kg -1 PGE 2 2 hours before the induction of ANP and for post intervention group of PGE 2,the same dose was administered right after the model was introduced. The serum levels of IL 10,IL 1β and TNF α in rats were measured by specific enzyme linked immuno sorbent assay(ELISA) at different points of time(1 h,3 h,6 h,12 h).Results:The serum IL 10 levels significantly enhances both pre intervention group(3 h) with PGE 2 and post intervention group(6 h) with PGE 2 compared with control group(ANP),(P<0.001,respectively). Serum TNF α and IL 1β levels of both groups treated with PGE 2 compared with control group(ANP) at different time points(1 h,3 h,6 h,12 h) were significantly reduced(P<0.001,respectively). Furthermore,IL 10 was positively related to TNF α(r=0.585,P<0.01) in ANP group,but negatively related in PGE 2 treatmented groups(r=-0.045,P<0.01;r=-0.143,P<0.01,respectively).Conclusion:The present study suggests that both pre intervention or post intervention with PGE 2 can enhance IL 10 levels,which potentially down regulated TNF α and IL 1β levels in rats.