摘要
本实验对放烧复合伤和单烧的大鼠于伤后24小时切痂植异体皮,观察术后不同时间皮肤组织学和超微结构变化。单烧大鼠的异体皮在术后8天开始排斥,19天已全部排斥。放烧大鼠的异体皮在术后29天观察期间多数存活,根据各时相点观察结果,术后1～4天为退变期,9～14天为修复生长期,24～29天为连续生长期,其存活较单烧时明显延长。
Skin allografts were transplanted to rats inflicted with third degree burns of 15% of BSA alone or combined with 5 Gy γ ray radiation.Histopathological,histochemical and ultrastructural changes of the allografts were examined at different postoperative periods. In burned rats,allografts began to be rejected on the 8th postoperative day,showing degeneration and necrosis of its epithelial cells.Only in one rat it survived on the 14th day(10%),and all allografts were rejected before the 19th day.In contrast to this,most allografts(60—80%)on the rats with combined injury survived well for a period of 29 days. Epithelial cells and collagen fibers of the allografts degnerated in 1 to 4 days postoperation, and recovered to near normal during 9 to 14 days.During 24 to 29 days postoperation,the epithelial cells and appendices appeared almost normal in texture except an infiltration of a few lymphocytes and monocytes.The restoration of degenerated collagen,however,was slower than that of degenerated epithelium.The result shows that the survival time of skin allografts in severe combined radiation burn injury is prolonged than that in burns.
