摘要
本实验应用光烧伤大白鼠背部(占体表面积20%Ⅲ度烧伤)动物模型进行研究烧伤后自由基、脂质过氧化对红细胞(RBC)损伤和抗氧化机制的变化。结果发现在光烧伤后,血浆过氧化脂质(LPO)增加,血浆GSHpx降低;RBC GSHpx活性在开始时增加,当不足以抵抗损伤时,抗氧化功能如G6PD、GSH、NPSH、GSHpx等活性降低,RBC LPO增加,蛋白SH受到损防,膜受损严重。总之,本实验证明了烧伤后RBC损伤机制为自由基和脂质过氧化所致,阐明了烧伤后RBC抗氧化功能的变化规律,提出需要深入研究以阐明自由基在烧伤后RBC内外产生的部位和引起这种变化的信息传递。
Thermal burn on the back of rat(20%TBSA fall-thicknesss burn)was used to study the changes in free radicals and lipid peroxidation and function of the antioxidation of erythrocy- tes postburn.It was found that there were increase in the concentration of lipid peroxides and decrease in activity of glutathione peroxidase(GSHpx)in plasma.The activity of RBC GSHpx increased significantly on day 1 postburn.When RBC GSHpx could not resist the in- jury,the function of antioxidation decreased,such as the activities of G6PD,GSHpx and the concentration of GSH and NPSH.RBC LPO increased,which cross-linked with sulfhydryl proteins and inflicted injury to RBC membrane.It might be concluded that the mechanism of damage of RBC after burn injury is due to chain reactions of free radicals and lipid peroxida- tion of the cell membrane.It also proved that there were changes in functional capability of antioxidation in vivo after burn injury.Further study is needed to locate the site of produc- tion of free radicals within or/and in the neighborhood of RBC and elucidate the transfer of the messages which cause the change.
