麦洼牦牛的RAPD分析

RAPD Analysis of Maiwa Yak

用60个10碱基的随机引物对麦洼牦牛的基因组DNA进行了PCR扩增。有7个引物扩增出了清晰且具多态性的条带,条带总数为34,其中有25条为可变条带,表现出多态性,多态频率为74%。根据Shannon公式计算出的7个引物扩增带频率的群体遗传多样性指数分别为:1.7955(OPA10)、0.6726(OPA13)、0.5433(OPB07)、0.0739(OPB10)、0.9919(OPA04)、1.4015(OPC06)、1.4514(OPK12),平均为:0.99。其平均百分比差异均值(MAPD)为40.41%。

PCR amplification was conducted for genome DNA of Maiwa yak using60random primers of10bp.7primers amplified the clear polymorphic bands.Total number of bands are34,and25bands are polymorphism.The rate of polymorphism is74%.The population genetic diversity index were1.7955(OPA10),0.6726(OPA13),0.5433(OPB07),0.0739(OPB10),0.9919(OPA04),1.4015(OPC06)and1.4514(OPK12)respectively.The MAPD(mean average percentage difference)was40.41%.