用MAB-ELISA对仔猪母源猪瘟抗体的试验

New-Bom Pigs Hog Cholera Antibody are Determinated by MAB-ELISA Test

用猪瘟兔化弱毒苗4头份免疫后的母猪3头,分别于分娩后定期日龄采血分离24头份血清样品;初生仔猪16头,分别于出生后定期日龄前腔静脉采血分离128头份血清样品,用单抗酶联免疫吸附试验检测血清猪瘟抗体滴度。结果表明,母猪猪瘟抗体效价持续较高,达100%保护率;哺乳前仔猪抗体效价为0,吃初乳后抗体水平不断提高,但抗体总体效价较低,仅有57.89%的保护率;仔猪20日龄前母源抗体水平差异较大,与母猪抗体水平不呈正相关。

The sows innunized with swine fever lapinized sucking vaccine were inspected hog cholera antibody by nonoclonal enzyme lined innunosorbent assay test.In the experlment,twenty-four serun specimens and one hundred twenty eight new-bom pigs were used.According to the results,it was proved that of sows hog cholera antibody were so high that100%protection rate,white of new-hom pigs hog cholera antibody were so low that barely57.89%protection rate sows antibody leval were no direct ralation.Now the experiment conditions wered as follows.