伪狂犬病病毒闽A株gE基因表位抗原编码区的克隆与序列测定

Cloning and Sequencing of gE Gene Fragment Encoding Epitopes of Pseudorabies Virus Fa Strain

用PCR方法以伪狂犬病病毒闽A株的基因组DNA为模板扩增到了gE基因表位抗原决定域的编码区段。PCR产物连接到 pMD18-T载体后 ,转化大肠杆菌XL 1-blue菌株。重组质粒经测序并与GenBank中已登录的gE基因序列比较 ,确证含有PRV闽A株 gE基因的表位抗原编码区。此区段与PRVRice株相应区段的DNA序列同源性为 97 3% ,氨基酸序列同源性为 94 7%。

The gE gene fragment encoding epitopes of pseudorabies virus Fa strain was amplified by PCR.This PCR product was cloned into pMD18-T vector and then sequenced.The sequencing result showed that the recombinant plasmid contained the epitopes encoding area of PRV Fa strain.The identity of DNA sequence and amino acid sequence of this region between PRV Fa strain and Rice strain was 97.3% and 94.7% respectively.