板栗种子失水过程中胚根细胞的亚显微结构变化

STUDIES ON ULTRASTRUCTURAL CHANGES OF RADICLE-TIP CELLS OF CHINESE CHESTNUT(Castanea mollissime Blume) SEED DURING DEHYDRATION

对板栗种子失水过程中的胚根细胞亚显微结构进行观察,结果表明,无论是人工加速脱水还是自然脱水,都可导致胚根细胞亚显微结构发生变化:即发生了质壁分离、质膜破碎、染色质均质化、内质网畸变和空泡化等现象。试验还表明,当接栗、灰栗、毛栗自然脱水量为44.7%,40.2%和39.0%时,其发芽率分别降至23.0%,5.2%和12.5%。在生产中应用时,应保持比上述含水量更多一些的水分。

Observation on the ultrastructure of the radicle-tip cell of Chinese chestnut seed indicates that either artificial accelerated dehydration or natural dehydration can cause ultrastructural changes, such as segregation of cytoplasm from the cell wall, fragmentation of cytoplasm membrane, homogenization of chromatin, malformation of endoplasmic reticulum and vesiculation of protoplasm. When the moisture content of Jeili, Huili, and Maoli decreases to 44.7%, 40.2%, and 39.0% in the process of natural dehydration, the germination percentege falls to 23.0%, 5.2%, and12.5% respectively. In production more moisture content than above must be kept in order to maintain the viability of the seed.