香菇多糖对巨噬细胞一氧化氮合酶活性和还原型谷胱甘肽的影响

Effects of lentinan on the inducible nitric oxide synthase and intracellular glutathione in mouse peritoneal macrophages

目的 研究香菇多糖 (LTN)对巨噬细胞诱导型一氧化氮合酶 (i NOS)活性和细胞内还原型谷胱甘肽 (GSH)水平的影响及其关系 ,探讨 L TN的免疫调节作用机制。方法 1观察 LTN诱导小鼠腹腔巨噬细胞 i NOS活性和细胞内 (GSH)水平的影响 ;2观察 m RNA转录抑制剂、蛋白质合成抑制剂和 NOS抑制剂对巨噬细胞 i NOS活性和细胞内 GSH水平的影响 ;3观察 GSH去除剂对巨噬细胞 i NOS活性的影响。结果 1 LTN诱导小鼠腹腔巨噬细胞 i NOS活性增加和细胞内 GSH减少 ;2 3种抑制剂抑制 L TN诱导的小鼠腹腔巨噬细胞 i NOS活性增加和细胞内 GSH减少 ;3GSH去除剂抑制 LTN诱导的巨噬细胞 i NOS活性。结论 LTN能增加小鼠腹腔巨噬细胞 i NOS的活性 ,并同时消耗细胞内的 GSH,提示细胞内GSH可能起到调节巨噬细胞 i NOS活性和保护宿主细胞免受 NO介导的细胞毒作用。

Objective To investigate the effects of Lentinan on the activity of inducible nitric oxide synthase(iNOS) and intracellular glutathione (GSH) in mouse peritoneal macrophages and to study the immunoregulation mechanisms of Lentinan.Methods (1) Observed the effects of Lentinan on iNOS and intracellular GSH in mouse peritoneal macrophages. (2) Observed the effects of mRNA transcriptional inhibitor Actinomycin D, protein synthesis inhibitor Actidione and NO synthase(NOS) inhibitor N-monomethyl-L-arginine(L-NMMA) on the activity of iNOS and intracellular GSH in mouse peritoneal macrophages. (3) Observed the effects of GSH eliminants BrO?ETA and BSO on the activity of iNOS. Results The results indicated:(1) Lentinan caused the increase of activity of iNOS in mouse peritoneal macrophages and decrease of intracellular GSH.(2)The effects were blocked efficiently by three inhibitors. (3)The effect of Lentinan on the activity of iNOS was inhibited by GSH eliminants.Conclusions It concludes that Lentinan can induce the increase of the activity of iNOS accompanied by depleted of intracellular GSH in the activated mouse peritoneal macrophages . It suggested that intracellular GSH plays an important role in regulation of iNOS activity and protects host cells from cytotoxic attack by NO.