四逆汤对心肌缺血大鼠心肌内皮素影响的实验研究

Experimental Study on Effect of Sini Decoction on Myocardial Endothelin in Myocardial Ischemic Rats

目的 :研究四逆汤对心肌缺血大鼠心肌内皮素 (ET)影响的分子机制。方法 :将SD大鼠随机分为对照组、缺血组和四逆汤组 ,用垂体后叶素造模 ,分别测定各组心肌ET浓度并做心肌ET 1的免疫组化及基因表达。结果 :四逆汤组心肌ET浓度明显低于缺血组 (P <0 0 1)。免疫组化显示ET 1主要定位在心肌细胞和血管内皮细胞 ,缺血组心肌ET 1染色灰度值明显低于对照组和四逆汤组 (P <0 0 1) ;RT PCR结果显示缺血组PCR产物条带灰度值显著高于对照组和四逆汤组 (P <0 0 1)。结论 :四逆汤可显著降低缺血心肌ET浓度 ,这可能与四逆汤能有效抑制缺血心肌ET 1基因的表达及蛋白合成有关。

Objective: To study the molecular mechanism of effect of Sini Decoction (SND) on myocardial endothelin (MET) in myocardial ischemic rats. Methods: Sprague Dawley rats were randomly divided into 3 groups, the normal control group, the ischemia group and the SND group. Myocardial ischemia was produced by pituitrin in the latter two groups. The content, immunohistochemical assay and gene expression of MET 1 were determined in all the three groups and compared. Results: The content of MET in the SND group was significantly lower than that in the ischemia group (P<0 01). Immunohistochemical examination showed that MET 1 was mainly located at the cardiac muscle cells and vascular endothelial cells with the grey scale obviously lower in the ischemia group than that in the control group and the SND group (P<0 01). While RT PCR showed that the grey scale of PCR product band in the ischemia group was significantly higher than that in the other two groups (P<0 01). Conclusion: SND could significantly lower MET content, it may be related to the effect of SND in inhibiting MET 1 gene expression and protein synthesis.