生长抑素类似物对结肠癌治疗作用的实验研究

Study on the analogue of Somatostatin for colorectal cancer

目的 :探讨生长抑素 (SST)类似物奥曲肽 (OCT)和NC 8 12 (生长抑素II型受体特异激动剂 )是否能在体内、体外实验中抑制结肠癌细胞的增殖。方法 :(1)采用MTT法对 2株结肠癌细胞株HCT116、LOVO进行不同生长抑素类似物作用下体外细胞增殖状况的研究。 (2 )于裸鼠皮下接种结肠癌细胞株HCT116后 ,皮下给予OCT和NC 8 12治疗 ,进行体内细胞增殖状况的研究。结果 :(1)体外生长实验的结果显示 :10 8mol/L的OCT或NC 8 12作用HCT116、LOVO细胞株 2 4h后 ,能明显抑制胰岛素引起的肿瘤细胞增殖作用。 (2 )治疗HCT116裸鼠移植癌的实验结果 :OCT和NC 8 12以 30 0 μg/ (kg .d)的剂量皮下给药共 2 1d的条件下 ,对肿瘤的生长 (以肿瘤的体积和最终瘤重为观察指标 )产生显著抑制作用。结论 :生长抑素类似物OCT和NC 8 12在体内、体外实验中均能抑制结肠癌细胞的增殖。

Objective:To confirm the analogue of Somatostatin OCT and NC 8 12 on the colon tumor cell growth in vitro and vivo.Methods:(1)Two colon cancer cell lines HCT116,LOVO of which cell proliferation was measured with MTT were treated with different analogue of Somatostatin in vitro.(2)The nude mice were implanted s.c.with colon cancer cell line HCT116,then were injected s.c. with OCT and NC 8 12.Results:(1).Cell proliferation of HCT116 induced by Insulin was obviously inhibited by OCT or NC 8 12 at 10 -8 mol/L 24h later after the cells were incubated with them.(2)The nude mice bearing xenografts of colon cancer HCT116 were treated for 21days subcontinuously with OCT?NC 8 12μg/(kg.d)],the inhibitory effect was seen obviously.,the inhibitory effect was seen obviously.Conclusion:The Somatostatin analogues OCT and NC 8 12 can inhibit the proliferation of colon cancer cells in vitro and vivo directly.