中性粒细胞与白细胞介素8在肺间质纤维化中的作用

Role of neutrophils and interleukin-8 in pathogenesis of interstitial lung fibrosis

目的 探讨中性粒细胞与白细胞介素 8(IL - 8)在肺间质纤维化发病中的的作用。方法 采用双抗体夹心酶联免疫吸附法 (EILSA) ,检测了 6例特发性肺间质纤维化患者 (IPE)和 7例对照者血清、支气管肺泡灌洗液 (BALF)中IL - 8水平 ,同时检测了BALF细胞总数及分类。结果 IPF组BALF中细胞总数及中性粒细胞明显高于对照组 (P <0 .0 1,P <0 .0 1) ,血清及BALF中IL - 8亦明显高于对照组 (P <0 .0 5 ,P <0 .0 1) ,且血IL - 8、BALFIL - 8水平与BALF中性粒细胞呈正相关 (r =0 .885 1,P <0 .0 5 ;r=0 .90 10 ,P <0 .0 5 ) ,血IL - 8与BALFIL - 8亦表现正相关 (r=0 .910 5 ,P <0 .0 5 ) ,在IPF组血IL - 8、BALFIL - 8与DLco呈负相关 (r=- 0 .714 1,P <0 .0 5 ;r=- 0 .72 34,P <0 .0 5 ) ,与血PaO2 亦呈负相关 (r=- 0 .86 2 1,r=- 0 86 11,P<0 .0 5 )。结论 IL - 8与中性粒细胞在特发性肺间质纤维化中起重要作用 。

Objective To investigate the role of neutrophils and interleudin 8(IL 8) in pathogenesis of interstitial lung fibrosis.Methods The serum and bronchoalveolar lavage fluid(BALF) samples were obtained from six patients with idiopathic pulmonary fibrosis(IPF) and seven controls.IL 8 levels in serum and BALF were determined by double antibody sandwich enzyme linked immunosorbent assay.Total and differential blood counts in BALF were examined.Results Total white cell counts and percentage of neutrophils in BALF of IPF patients were significantly higher than those in control group(P<0.01,P<0.01).IL 8 was significantly elevated in serum(246.3±72.4 μg/L,P<0.05) and BALF (657.8±116.0 μg/L,P<0.01) of patients with IPF,compared with control group(IL 8 in serum,168.5±23.6 μg/L;IL 8 in BALF,296±87.3 μg/L).The levels of IL 8 in serum and BALF of patients with IPF were positively correlated with the percentage neutrophils in BALF(r=0.8851,r=0.9010,P<0.05).Furthermore,a significant positive correlation between serum IL 8 and BALF IL 8 was observed (r=9105,P=0.05).The levels of IL-8 in serum and BALF showed a negative correlation with DLco(r=-0.7141,r=-0.7234,P<0.05) and PaO 2 (r=-0.8621,r=-0.8611,P<0.05),respectively.Conclusions Neutrophils and IL 8 might play an important role in pathogenesis of IPF and can estimate the degree of alveolitis in patients with IPF.