硝酸纤维素膜印迹法测定对虾微量血淋巴碱性磷酸酶相对活性

<title>Microdetermination of relative activity of ALP in hemolymphof shrimps by dot blot on nitrate cellulose membrane (NCM)

用硝酸纤维素膜(NCM)印迹法测定日本对虾(Penaeusjaponicus)血淋巴碱性磷酸酶(ALP)的相对活性,以标准ALP梯度稀释液为参照,比较NCM印迹法与ALP测定试剂盒法对标准ALP梯度液的测定结果。数据回归分析显示,NCM印迹法所得结果同标准ALP梯度稀释液活性的回归关系极显著(P<0.01);用NCM印迹法测定ALP相对活性的灵敏度为试剂盒的100倍。分别用NCM印迹法与ALP测定试剂盒测定对虾血淋巴的ALP相对活性,测得结果呈强直线相关性(P<0.01),说明用NCM印迹法测定对虾血淋巴ALP相对活性具可行性。

<Abstrcat> The hemolymph was collected from cultured shrimp Penaeus japonicus and nitrate cellulose membrane (NCM) was used for dot blot to test the relative activity of ALP in the hemolymph , and meanwhile the commercialized standard ALP kit was employed to conduct a reference test. The regress analysis on the test results shows that the data from the measurement of dot blot on NCM have a remarkable linear correlation with that from the reference test (P<0. 01). The sensitivity of ALP measurement by NCM dot blot is 100 times as much as that of the reference test (by ALP kit). The test results of activity and relative activity of ALP in the hemolymph have a close linear correlation (P<0.01).