离子束诱变技术在海洋微藻培育中的应用

<title>Marine microalgae culture by ion beam implantation

以球等鞭金藻Isochrysisgalbana3011(简称微藻3011)为材料,对其进行离子注入诱变体系的建立及注入后的生物学效应的初步研究。结果表明,用质量分数为10%的甘油培养液处理微藻3011,并收集藻泥均匀涂布于灭菌后的滤纸上,风干后进行离子注入,效果最为理想。不同剂量的N+注入后,微藻3011存活曲线呈先降后升再降的"马鞍型",即当N+注入量为(0～9.1)×1014ions/cm2时,存活率随注入剂量的增大而迅速下降;当N+注剂量为(9.1×1014)～(1.872×1015)ions/cm2时,存活率随注入剂量的增大而有所上升,但远低于对照组;当N+注入剂量增大到1.872×1014ions/cm2时,其存活率又开始逐渐下降。此结果与紫外线、γ-射线等其他射线辐照生物有机体存活曲线呈"肩型"或"直线型"不同,但与离子注入陆地生物有机体后的存活曲线相近。本研究旨为探讨离子束生物技术应用于海洋生物体遗传改良中的可行性,并为之提供科学依据。

<Abstrcat> Isochrysis gablana 3011 samples were used as the experiment materials to establish the ion beam implantation system. The strain 3011 was treated in 'f/2' based culture medium containing glycerine (5%, 10%, 15%, 20%) or DMSO (0.5%, 1%, 2%, 4%) as the protective additive and the protective effect was the best with 10% glycerin. With the implantation of N+, the survival rate of strain 3011 declined at the first until the N+ dose increased to 9.1×1014 ions/cm2, i.e. the survival rate of strain 3011 got down rapidly with the increase of N+ dose; but when N+ dose increased from 9.1×1014 to 1.872×1015 ions/cm2, the survival rate went up with the increase of N+ dose, but was much lower than that in control (vacuum, without N+ implantation, 100% survival rate); when N+ dose increase to over 1.872×1015 ions/cm2, the survival rate began to decline with the N+ dose increase. So, the survival rate curve shows 'saddleshaped', which is much different from the 'shouldershaped' one or 'straightlineshaped' one by γray, ultraviolet ray or other radiation sources. The purpose of this study is to research the possibility of ion beam implantation used in plasma modification of marine organisms and to provide reliable basic data on that. 