摘要
通过对紫花斜茎黄芪 (Astragalusadsurgens)进行组织培养和植株再生 ,着重对培养基和培养条件等方面进行了筛选和研究 ,结果表明 ,紫花斜茎黄芪腋芽和茎尖作为外植体的组织培养和继代增殖培养 ,最适宜培养基为MS + 1mg/LBA + 0 .1mg/LNAA和MS + 0 .5mg/LBA + 0 .1mg/LNAA。培养温度 2 0～ 2 6℃ ,光照每天 10h ,光照强度 15 0 0Ix。生根培养最适宜的培养基为MS + 0 .1mg/LNAA ,培养温度 2 0～ 2 5℃ ,光照每天 10h ,光照强度仍为 15 0 0Ix。
The tissue culture with the axillary buds and apicillary buds showed that the best medium for Astragalus adsurgens tissue culture is MS+1 mg/L BA+0.1mg/L NAA and MS+0.5mg/L BA+0.1 mg/L NAA. The temperature for the culture is 20-26 ℃with 10 hours of illumination per day and 1 500 lx of illumination intensity. The best medium for rooting is MS+0.1 mg/L NAA with 25 ℃ temperature, 10 hours of illumination per day and 1 500 lx illumination intensity.
出处
《草原与草坪》
CAS
2002年第3期49-50,共2页
Grassland and Turf
基金
甘肃省科技厅项目"野生豆科牧草与紫花科茎黄芪选育研究"部分内容
