吸入低浓度一氧化氮治疗高原肺水肿对体内自由基代谢的影响

EFFECTS OF NO ON FREE REDICAL METABOLISM IN TREATMENT OF HIGH ALTITUDE PULMONARY(HAPE)

研究吸入低浓度一氧化氮 (NO)治疗高原肺水肿对体内自由基代谢的影响 ;方法 :在海拔 3 70 0m采用吸入低浓度 (0 .0 0 1% )NO 6 0分钟治疗 8例高原肺水肿患者 ,在治疗前吸入NO 5 0分钟即刻和治愈后分别采肘静脉血检测红细胞超氧化物歧化酶(RBC -SOD)、丙二醛 (MDA)、全血和血浆谷胱甘肽过氧化物酶 (GHS -Px)的含量 ;结果 :高原肺水肺患者吸入NO治疗 5 0分钟即刻较治疗前RBC -SOD和MDA降低 ,差异显著 (P <0 .0 5 ) ,全血和血浆GHS -Px活性水平增高 ,差异显著 (P <0 .0 5 )。治愈后较吸入NO 5 0分钟即刻RBC -SOD降低 ,有显著性差异 (P <0 .0 5 ) ,MDA降低 ,差异非常显著 (P <0 .0 1) ,GHS -Px均增高 ,差异非常显著 (P <0 .0 1)。治愈后较治疗前RBC -SOD和MDA降低 ,有非常显著性差异 (P <0 .0 1) ,GHS -Px活性水平均增高 ,差异非常显著 (P <0 .0 1) ;结论 :吸入 0 .0 0 1%NO一小时增强了抗氧化酶GHS -Px的活性水平 ,加速了体内氧自由基的清除作用。

To study the effects of NO on free redical metabolism in treatment of high altitude pulmonary edema(HAPE).Methods:Level of RBC-SOD,MDA,GHS-Px were measured before and in NO(0.001% concentration)being inhalated 50 minutes,same perform after recovery,in 8 patients with HAPE.Results:The level of RBC-SOD and MDA in inhalating NO 50′ were lower (P<0.05);and the level of GHS-Px was highes (P<0.05) than that before NO inhalating.Same results (P<0.01) as in recovery compared with NO inhalation. Conclusions:The activity of GHS-Px increase 1hr after NO (0.001% concentration) inhalation to eliminate the more free redical in patients with HAPE.