大承气汤对肠梗阻大鼠离体结肠平滑肌^(45)Ca内流影响的实验研究

Study of Da Cheng Qi Tang(大承气汤)on ^(45)Ca Content of the Isolated Colon Smooth Muscle from Experimental Colon Obstruction Rats

本实验观察了正常对照组和实验性结肠梗阻组大鼠离体结肠平滑肌(45)~Ca 的内流,并研究了大承气汤对实验性结肠梗阻大鼠离体结肠平滑肌(45)~Ca 内流的影响。结果表明:结肠梗阻组大鼠结肠平滑肌(45)~Ca 内流显著增加,大承气汤能明显抑制梗阻结肠(45)~Ca 内流,对正常结肠平滑肌(45)~Ca 内流无明显影响。提示肠梗阻的发生与发展和平滑肌内 Ca^(2+)浓度升高有一定关系。大承气汤抑制梗阻结肠平滑肌(45)~Ca 内流增加可能是该方剂治疗息性肠梗阻的离子机制之一。

Da Cheng Qi Tang(DCQT)is a classical prescription of Chinese medicine for treatment of acute intestinal obstruction.In this paper,the isolated colon smooth muscle from normal and experimental colon obstruction(CO)rats were used to study the effect of DCQT on(45)~Ca content.The results showed that the(45)~Ca content on isolated colon smooth muscle(μm/g wet tissue,(?)±S)was 0.043 ±0.009 in the normal and 0.057±0.012 in those treated by DCQT respectively.The content of(45)~Ca in CO was higher than the normal,DCQT can reduce the content of(45)~Ca in CO.It is known that the higher level of intracellular Ca^(++)is related to the formation and the development of acute intestinal obstruction.The inhibitory effect of DCQT on(45)~Ca content may play an important role in the treatment of acute colon obstruction.