广东汉族人群细胞因子基因多态性研究

A study of the distribution of cytokine gene polymorphisms of Han in Guangzhou.

目的 探讨细胞因子基因多态性分布。方法 采用PCR -SSP对 5 8名随机人群的肿瘤坏死因子、转化生长因子、IL - 6、IL - 10、干扰素等细胞因子分别作基因多态性研究。结果 肿瘤坏死因子三个基因型频率分别为 0 4 31、0 4 31、0 138,等位基因频率G =0 6 4 6、A =0 35 4 ;转化生长因子九个基因型频率分别是 0 0 6 9、0 0 5 2、0 172、0 0 17、0 190、0 190、0 0 34、0 0 6 9、0 2 0 7,等位基因频率T =0 5 0 9、C =0 4 91、C =0 2 76、G =0 72 4 ;IL - 6三个基因型频率分别是 0 2 0 4、0 6 38、0 15 5 ,等位基因频率C =0 2 5 9、G =0 74 1;IL - 10六个基因型频率分别是 0 0 86、0 10 3、0 15 5、0 0 6 9、0 2 76、0 310 ,等位基因频率GCC =0 2 16、ACC =0 2 5 9、ATA =0 5 2 5 ;干扰素三个基因型频率分别是 0 5 5 1、0 310、0 138,等位基因频率A =0 70 7、T =0 2 93。结论 本组数据对细胞因子基因多态性研究有助于从分子水平去认识细胞因子在机体免疫调节的重要作用 ,对临床上某些疾病发病机理的研究、诊断和防治提供有力的依据。

Objective: To investigate the distribution of cytokine gene polymorphisms. Methods: TNF, TGF, IL-6, IL-10, IFN gene polymorphisms were typed using PCR-SSP in 58 people respectively. Results: The three TNF genotype frequencies were 0 431, 0 431, 0 138.The TNF allelic frequencies were 0 646(G), 0 354(A). The nine TGF genotype frequencies were 0 069, 0 052, 0 172, 0 017, 0 190, 0 190, 0 034, 0 069, 0 207. The TGF allelic frequencies were 0 509(T), 0 491(C), 0 276(C), 0 724(G) The three IL-6 genotype frequencies were 0 204,0 638,0 155.The IL-6 allelic frequencies were 0 259(C), 0 741(G) The six IL-10 genotype frequencies were 0 086, 0 103,0 155, 0 069, 0 276, 0 310.The IL-10 allelic frequencies were 0 216(GCC), 0 259(ACC), 0 525(ATA). The three IFN genotype frequencies were 0 551, 0 310, 0 138. The IFN allelic frequencies were 0 707(A), 0 293(T). Conclusion: These data indicate that investigating cytokine gene polymorphisms can help us to realize the importance of cytokine in immune modulation and study the pathogenesis, diagnosis, prevention and cure of some diseases.