利胆合剂降解大肠杆菌内毒素的电镜观察

Effect of Promoting Biliation Mixture on E.Coli under Electron Microscopy

采用电镜的方法观察了利胆合剂、多粘菌素 B 对 O_(111)B_4大肠杆菌内毒素的影响。结果表明:利胆合剂可将内毒素原具有很多分支的长链状结构裂解成短杆状或短片段,部分发生完全解聚。其形态学改变类似多粘菌素 B 的作用。结果提示,利胆合剂体外可使大肠杆菌内毒素发生一定程度的降解。本实验结果或许能为利胆合剂的临床疗效提供一定的理论依据。

With electron microscopy this article observed the effect of pronioting biliation mixture(PBM) and polymyxin B on E.coli lipopolysaccharide (LPS).The result showed that PBM could breakdown the typical structure of E.coli LPS with only short sections or partially disaggregate it.The morphology changes were similar to the effect of polymyxin B. It would appear that the loss of endotoxicity caused by PBM may be due to the loss of structural integrity of the E.coli LPS.The result may also give some evidence for the clinical effects of PBM theoretically.