摘要
本实验用离体豚鼠右心室乳头肌为标本,采用张力传惑器记录心肌收缩力,常规玻璃微电极细胞内记录动作电位,首次观察了熊脱氧胆酸(UDCA)对心肌收缩力及电活动的影响。结果表明:UDCA0.55mg/ml 作用30 min,可使心肌收缩力由给药前的143.5±25.9mg 降低至91.9±17.3mg(P<0.001),并可使动作电位时程缩短(n=3),与熊胆的作用一致。表明熊胆中对心肌电及机械活动作用的主要有效成分为 UDCA。本结果为 UDCA 代替熊胆治疗某些心血管疾病提供了实验依据。
Using electromechanical transducer and conventional microelectrodes in isolated guinea pig heart muscles,the effects of ursodeoxycholic acid(UDCA)on the mechanical and electric activities were firstly observed.The results showed that,UDCA in dose of 0.05 mg/ml decreased contractile force by 36%(P<0.001)and shortened action potential duration(n=3).It is inferred that UDCA may block inwared calcium current.The results suggest that the action of UDCA is similar to that of bear bile and may be an effective composition of bear bile which acts on heart muscle.Our findings provide the experimental basis for the clinical application of UDCA.
关键词
熊脱氧胆酸
心肌收缩
动作电位
ursodeoxycholic acid
papillary muscle
contractile force
action potential
