小麦糯性突变体的筛选

Mutant Screen of Wheat Cultivars(T.aestivum) for Waxy Genes(null)Types

利用SDS -PAGE技术 ,从河北省小麦地方品种及国内外小麦育成种 12 70份中 ,发现Wx B1突变体 6 3份 ,Wx D1突变体 4份 ,Wx E 2份。红蘖麦和白芒白等品种的发现 ,丰富了品质育种的亲本 ;长穗偃麦草的蜡质基因与小麦蜡质基因不同 ,可以进行更深入的分子水平研究。研究表明 ,地方品种和育成种具有相似的突变规律 ;国外优良品质材料含有较高的Wx B1突变频率 ;国内以源自北京、山西、陕西、云南的材料突变频率最高 ;河北、河南、山东、江苏等省份突变频率居中 ;而四川、天津、黑龙江和安徽等省份的样本量太小 ,突变频率也最低。该频率分布规律为今后育成种筛选的重点提供了帮助。

The technique of SDS PAGE was used for the waxy gene(null) mutant selection among 1 270 varieties from both landraces and breading lines of domestic and aboard origin.Sixty three Wx B1(null)varieties,4 Wx D1(null) mutants and 2 Wx E materials were successfully obtained.The discovery of hongniemai and baimangbai enriched breading parents and established a solid foundation for wheat quality breeding in China.This will enable us to create new waxy wheat cultivars.The waxy gene of Elytrigia elongata (HOST) . Beauv is different form that of Triticum aestivum and further study on molecular level should be conducted.The result indicated that landraces and breading lines follow the same mutant pattern.The frequency pattern provides us useful information in future mutant selection form breading lines.