河南省21个小麦品种(系)高分子量麦谷蛋白亚基的电泳分析

Electrophoresis Analysis on High Molecular Weight Glutenin Subunits(HMW-GS) of 21 Wheat Cultivars in Henan Province

用十二烷基硫酸钠—聚丙烯酰胺凝胶电泳 (SDS PAGE)对河南省 2 1个小麦新品种 (系 )麦谷蛋白高分子量 (HMW )亚基构成、不同等位基因间亚基变异及出现频率进行了系统分析 ,并计算了品质得分。结果表明 ,河南省现有小麦新品种 (系 )的高分子量麦谷蛋白亚基构成中以 2 + 12亚基居多 ,5 + 10亚基的品种数量较少。对今后河南省小麦品质改良提出了建议。

The grain proteins of 21 wheat cultivars in Henan province were fractionated by SDS-PAGE to determine their high molecular weight glutenin subunit composition,allelic variation and frequencies.The quality scores of all the subunits were summed to create a GLU 1 quality score for each cultivars.The result indicated that the frequency of subunits 5+10 was low in Henan province grown wheat cultivars.The results in relation to future strategies for improving quality of Henan wheat cultivars were discussed.