胰岛素、地塞米松、甲基异丁基黄嘌呤对脂肪细胞分化过程及PAI-1基因表达的影响

Insulin, Dexamethasone and Methylisobutylxanthine Regulate PAI-1 Gene Expression in 3T3-L1 Adipocytes

目的 通过体外培养的 3T3 L1细胞分化模型 ,研究胰岛素 (Ins)、地塞米松 (Dex)和甲基异丁基黄嘌呤(Mix)对脂肪细胞分化过程及纤溶酶原激活剂抑制剂 1(PAI 1)基因表达的影响。方法 观察Ins与Dex及Mix加入前后脂肪细胞形态的变化 ,并应用RT PCR方法 ,在不同浓度的Ins、Dex和Mix及其不同组合的条件下 ,对3T3 L1细胞分化为脂肪细胞过程中PAI 1mRNA表达水平进行相对定量分析。结果 Ins与Dex及Mix显著促进了 3T3 L1细胞向脂肪细胞分化 ,Ins、Dex及其组合Ins加Dex对PAI 1基因的表达有促进作用 ,其中尤以0 .15 μmol/LIns加 0 .1μmol/LDex的作用最为显著 ,且有叠加效应。 结论 Ins与Dex加Mix对 3T3 L1细胞分化为脂肪细胞起重要作用 ,同时Ins、Dex对PAI 1基因表达也有显著影响 。

Purpose. To investigate the plasminogen activator inhibitor-1 (PAI-1) gene expression in mouse 3T3-L1 adipocyte and the differentiation of 3T3-L1 cells in vitro. Methods. The morphological changes in 3T3-L1 cells during differentiation has been observed, and the relatively quantitative RT-PCR was used to research the effect of insulin (Ins), dexamethasone (Dex) and methylisobutylxanthine (Mix) acting alone or in combination on PAI-1 mRNA production. Results. The findings demonstrated that Ins, Dex in combination boosted the significant increase in PAI-1 mRNA and acted synergistically at a certain concentration, suggesting that Ins and Dex stimulate the different signaling pathway in different way, and that the factors triggered in the two pathways may interact during the signaling process. Conclusions. Ins, Dex and Mix play a essential role in induction of 3T3-L1 cells, and Ins, Dex dramatically elevate the increase in PAI-1 mRNA level in fully differentiated adipocyte. These provide evidence to further establish a mechanism through which Ins and Dex regulate the PAI-1 gene expression in 3T3-L1 adipocytes.