CpGDNA预防小鼠哮喘模型的形成

An Experimental Study of the Prevention of CpG DNA on the Production of a Murine Asthmatic Model

目的 研究含胞嘧啶鸟嘌呤核苷酸的免疫刺激元件 (CpGDNA)能否预防卵蛋白致敏小鼠哮喘模型的形成。方法 将 18只BALB/c小鼠均分为 3组 ,其中卵蛋白致敏哮喘组 (OVA组 )和CpG预防组 (CpG组 )皮下注射卵蛋白致敏制作哮喘模型 ,然后用卵蛋白激发 2次 ;阴性对照组 (NS组 )皮下注射生理盐水 ,然后生理盐水激发 2次。CpG组在致敏前腹腔注射CpGDNA 10 0 μg/ 10 0 μL ,其他两组不作干预。 3组分别在第 2次激发后 2 4h测外周血OVA特异性IgE、IgG1、IgG2a,并处死小鼠测肺泡灌洗液 (BALF)中的细胞总数及嗜酸性粒细胞 (EOS)计数 ,肺组织病理切片观察形态学改变 ,并测定脾细胞培养上清液中OVA特异性IFN γ。结果 CpG组BALF中细胞总数和嗜酸性粒细胞明显低于OVA组 ,P <0 .0 5 ;CpG组肺组织炎症反应较OVA组明显减轻 ;CpG组脾细胞培养上清液中OVA特异性IFN γ的浓度明显高于OVA组 ,P <0 .0 5 ;CpG组外周血OVA特异性IgE和IgG1均低于OVA组 ,P <0 .0 5 ;CpG组IgG2a较OVA组为高 ,P <0 .0 5。

Purpose. To investigate the prevention of CpG DNA on the production of a murine asthmatic model. Methods. 18 Female BALB/c mice were divided into 3 groups. Group CpG and group ovalbumin(OVA) were sensitized subcutaneouly with OVA/alum and challenged with OVA. Group NS were injected and challenged with NS. Group CpG was injected CpG DNA 100 g/100 μL intraperitioneally prior to sensitization. Blood samples, bronchoalveolar lavage fluid(BALF), lung tissues and spleen cells were collected one day after challenge. Serum OVA-specific IgE, IgG1, IgG2a were measured by ELISA. Total cell numbers and classification of BALF were counted. IFN-γ producted by OVA-stimulated spleen cells was determined by ELISA. Results. Pathological examination showed that in group CpG airway inflammation and eosinophil infiltration in airway and lung parenchyma were inhibited as compared with group OVA. In group CpG total cell numbers and Eosinophilia of BALF were decreased, serum levels of IgE and IgG1 were decreased, serum level of IgG2a and the level of IFN-γ in spleen cell supernatants were increased as compared with group OVA. Conclusions. CpG DNA could prevent the production of a murine asthmatic model.