IMMULITEⅠ型全自动分析系统使用初评

A Primary Appraising for IMMULITEⅠAutomated Immunoassay System

本文通过测定血清中T3 ,T4含量为例 ,介绍IMMULITEⅠ型酶放大化学发光免疫分析仪及化学发光法与放射免疫分析法 (RIA)的比较。IMMULITEⅠ型分析仪测得血清中T3 ,T4重复性良好 ,批内变异和批间变异均小于 5 %。在对T3 值是 4 32ng/dL和 377ng/dL的血清样品作回收实验 ,回收率为 10 3.2 % - 116 .9%。两种方法测得T3 结果呈相关 ,相关系数 0 .91,回归方程为Y =1.0 6X +0 .15 9。IMMULITEⅠ型分析仪测量 ,在2 5min后出第一个样品结果 ,以后每 35sec出另一个结果 ,而RIA通常需要几小时或更长时间 ,才能出结果。因此IMMULITEⅠ型分析仪操作简便 ,其采用的化学发光法可与RIA媲美 ,是一种功能全 ,运行平稳的化学发光分析仪 。

To compare the 2 analytical methds, CIA(chemiluminescent immunoassay) and RIA, serum T 3 and T 4 concentrations were analyzed by IMMULITE Ⅰ chemiluminescent immunoassay system and RIA respectively. The intra-and inter-assay CV of CIA were smaller than 5%. The serum containing 432ng/dL and 377ng/dL T 3 was performed in the recovery test. The recovery rate was 103.2% to 116.9%. The analytical results of the two methods were highly correlated. The correlation coefficient was 0.91, and the regression equation is Y=1.06X+0.159. IMMULITE Ⅰ analysis system needs 25 min to have the first result and then every 35 seconds for one of the following sample results, but the RIA needs several hours to have the results. IMMULITE Ⅰ analysis system is based on CIA, its results are highly comparable with the RIA. Its stable, simple to operate and can be extensively used for clinical applications.