摘要
通过体外培养心肌细胞探讨磷脂酶 A2 (phospholipase A2 ,PL A2 )、脂多糖 (lipopolysaccharide,L PS)对心肌细胞膜的损伤及其磷酸氯喹 (chloroquine phosphate,CQP)对心机细胞膜的保护效应。实验结果表明 ,PL A2 、L PS在体外和培养心肌细胞一起孵育 ,能引起心肌细胞膜脂质过氧化损伤加重 ,降低细胞膜流动性 ,增加细胞膜通透性。L PS和体外培养心肌细胞一起孵育 ,可导致 PL A2 活性显著升高 ,细胞内脂质过氧损伤加重 ,细胞膜通透性增加 ,细胞膜流动性降低。而CQP可减轻心肌细胞膜的脂质过氧化损伤 ,抑制 PL A2 活性 ,改善细胞膜流动性 ,保护心肌细胞膜 。
In order to investigate the effects of PLA 2,LPS on myocardial membrane and the protective effects of CQP,the incubated myocardial cell in vitro were used.The experimental results indicated that myocardial cells incubated with LPS,PLA 2,myocardial membrane lipid peroxide was significantly increased,the membrane fluidity was lowered,the membrane permeability was strongly increased.Myocardial cells incubated with LPS,the PLA 2 activity significantly elevated,lipid peroxide injury was heavy,membrane permibility was increased,the membrane fluidity was lowered.The experiment showed:CQP can reduce lipid peroxida injury of myocardial cell membrane,can inhibit PLA 2 activity,CQP may improve the cell membrane fluidity,may prevent the damage of cell membrane,reduce the impairment of myocardial cell.
出处
《现代预防医学》
CAS
2000年第3期290-291,共2页
Modern Preventive Medicine
